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Dorsal thalamic axons (red) are confronted to a protein stripe choice assay for testing their preference to grow between lines coated with a candidate substrate (green lanes) and control lanes (black lanes). Coronal section of a Robo1-/-;Robo2-/- double mutant mouse at E18.5 showing the abnormal crossing of the midline of cortical axons after double (DiI red, DiA green) cortical injections. Nuclear staining is shown in blue. Oblique section of an E13.5 mouse brain showing thalamocortical axons (green) traversing the ventral telencephalon after 48 h in culture. Thalamocortical axons were labeled by electroporation of a plasmid codifying for Gfp in the dorsal thalamus. Oblique section of an E14.5 mouse brain showing double immunoreativity for Robo2 receptor (red) expressed in thalamocortical axons and Islet1 transcription factor expressed in corridor cells (green) at the ventral telencephalon.
βIII-tubulin immunohistochemistry showing thalamocortical axons from an E13.5 embryo after 48 h in culture in poly-L-lysine/laminin coverslips. High-power view of a coronal section through an E18.5 embryonic thalamus showing the topography of the thalamocortical interconnectivity after triple (DiI red, DiA green, DiI red) cortical injections. Nuclear staining is shown in blue. Dorsal thalamic neurons (red) backlabelled in the ventrobasal nucleus of the thalamus after a cortical injection of DiI in S1 at E18.5. Thalamocortical axons from an E13.5 Gfp-transgenic mouse (green) showing F-actin localization (red) in growth cones after 48 h in culture in poly-L-lysine/laminin coverslips.
High-power view of a double immunohistochemistry for Lhx2 transcription factor (green) and L1 (red) in the dorsal thalamus at E13.5. Nuclear staining is shown in blue. Coronal section of an E15.5 mouse showing the pathway of thalamocortical axons (green) after an in utero electroporation of a Gfp-codifying plasmid in the dorsal thalamus at E12.5. Nuclear staining is shown in blue.


GLB lab
Instituto de Neurociencias · UMH-CSIC
Campus San Juan · Alicante | España
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