HomeAbout the InstituteResearch UnitsPeopleLinksPh.D ProgramEnglish Language Idioma EspañolWeb SearchIntranet Access
 
 
Research highlights About the institute
A method for visualizing the intact Central Nervous System of mouse embryos in 3D with the greatest detail.

Phenotyping the central nervous system of the embryonic mouse by magnetic resonance microscopy.

Neuroimage (2014), online 22 April 2014

Maria Angeles Martínez-Martínez, Jesús Pacheco, Víctor Borrell and Santiago Canals

Genetic mouse models of neurodevelopmental disorders are being massively generated, but technologies for their high-throughput phenotyping are missing. The potential of highresolution magnetic resonance imaging (MRI) for structural phenotyping has been demonstrated before. However, application to the embryonic mouse central nervous system has been limited by the insufficient anatomical detail. Here we present a method that combines staining of live embryos with a contrast agent together with MR microscopy after fixation, to provide unprecedented anatomical detail at relevant embryonic stages. By using this method we have phenotyped the embryonic forebrain of Robo1/2-/- double mutant mice enabling us to identify most of the well-known anatomical defects in these mutants, as well as novel more subtle alterations. We thus demonstrate the potential of this methodology for a fast and reliable screening of subtle structural abnormalities in the developing mouse brain, as those associated to defects in disease-susceptibility genes of neurologic and psychiatric relevance.

 

Research Highlights Archive

CSIC-UMH
 
 
Consejo Superior de Investigaciones Científicas
Universidad Miguel Hernández

Campus de San Juan | Sant Joan d’Alacant
Alicante | España

in@umh.es
direccion.in@umh.es
Tel. + 34 965 23 37 00
Fax + 34 965 91 95 61
© 2004-2016 Instituto de Neurociencias
Alicante | España | Legal Note | Mapa Web
Diseño web Digital Nature